The Caspase-2 Substrate (VDVAD-AFC) assay is a highly sensitive method for detecting caspase-2 activity in apoptotic cells. This assay utilizes a fluorescent substrate (VDVAD-AFC) to monitor the cleavage activity of caspase-2, an enzyme involved in programmed cell death. By measuring fluorescence, researchers can gain valuable insights into the mechanisms of apoptosis, necrosis, and cell death in various disease models, making it an essential tool for drug discovery and cellular biology research.
